Optimization of Chaenomeles SRAP-PCR Reaction System through the Quadratic Orthogonal Rotatable Combinatorial Design

Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design.

The effects of 5 factors (template DNA, Mg(2+), dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymor-phism (SCoT)-PCR system of Dactylis glomerata L., using an orthogo-nal design L16 (4(5)). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction volume contained 3.0 mM Mg(2+), 0.2 mM...

Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi.

Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L16 (45) design was employed to investigate the effects of five factors (Mg2+, dNTPs, Taq DNA polymerase, primer, and template DNA) on the ...

Combinatorial Optimization through Multiresolution Analysis

Recently, multiresolution analysis and wavelets have had a great impact on computer algorithm design, chieey in the areas of signal processing and compression, computer graphics, and numeric optimization algorithms. Of the algorithms that have beneetted, Meyers mul-tiresolution polygon tiling algorithm is unique in that it solves a combinatorial optimization problem. This paper suggests a gener...

On combinatorial optimization problems arising in computer system design

Combinatorial Optimization in VLSI Design

VLSI design is probably the most fascinating application area of combinatorial optimization. Virtually all classical combinatorial optimization problems, and many new ones, occur naturally as subtasks. Due to the rapid technological development and major theoretical advances the mathematics of VLSI design has changed significantly over the last ten to twenty years. This survey paper gives an up...